Caisson Laboratories Plant Growth Regulators – Plant Tissue Culture Protocol

The importance of plant growth regulators in plant tissue culture is well documented. Caisson offers a broad range of plant growth regulators specifically tested for plant cell culture. Each
product is biologically tested following the criteria established for powdered media. Each auxin is tested for enhancement of callus growth and/or root initiation in vitro . Each cytokinin is tested
for stimulation of shoot production

The plant growth regulator solutions are sterile filtered through a 0.2 µm filter unit into sterile bottles. Each lot of plant growth regulator solution is tested for sterility according to specifications
established by U.S. Pharmacopeia, as well as biological testing in plant cell culture using criteria identical to those established for our plant tissue culture media.
FOR LABORATORY USE, PLANT TISSUE CULTURE MEDIA PREPARATION AND PLANT RESEARCH PURPOSES ONLY. NOT FOR USE AS A PLANT GROWTH REGULATOR ON
DEVELOPED PLANTS. NOT FOR DRUG OR HOUSEHOLD USES

Preparation and Use

To prepare a 1 mg/ml stock solution: Add 100 mg of the plant growth regulator to a 100 ml flask or other glass container. Add 2-5 ml of solvent to dissolve the powder. Once completely
dissolved, bring to volume with cell culture grade water (Catalog No. WAL01). Stirring the solution while adding water may be required to keep the material in solution. Store the stock solution
as recommended in the tables. Add 1.0 ml of the stock solution to 1 liter of medium to obtain a final concentration of 1.0 mg/L of the plant growth regulator in the culture medium. (See Table
below.)

Volume of Stock Solution = (Desired Hormone Conc. X Medium Volume) / Stock Solution Conc.

Plant Cell Culture Tested Auxins are generally used in plant cell culture at a concentration range of 0.01-10.0 mg/L. When added in appropriate concentrations, they may regulate cell
elongation, tissue swelling, cell division, formation of adventitious roots, inhibition of adventitious and axillary shoot formation, callus initiation and growth, and induction of embryogenesis.

Plant Cell Culture Tested Cytokinins are generally used in plant cell culture at a concentration range of 0.1-10.0 mg/L. When added in appropriate concentrations, they may regulate cell
division, stimulate axillary and adventitious shoot proliferation, regulate differentiation, inhibit root formation, activate RNA synthesis and stimulate protein and enzyme activity.

*CA = coautoclavable with other media components. F = filter sterlize. CA/F = coautoclavable with other media components,
however, some loss of activity may occur. This can be compensated for by increasing component concentration. Component may be
filter sterilized.

Catalog
No. Product Name Type Mol. Wt. Solvent Diluent
Powder
Storage
Liquid
Storage
Sterilization
Working
Conc.
(mg/L) Plant Effect
A036 (±)-cis,trans-Abscisic acid (ABA)
Plant
hormone 264.3
EtOH/1N
KOH Water -20°C -20°C CA/F 0.1-10.0 No growth, turns brown
C043
2-carboxyphenyl 3-phenylpropane
1,3-dione (CPD)
Auxin
Inhibitor 268.26 Water Water 2-8°C -20°C CA/F 0.1-10.0 Auxin inhibitor
D013
3,6-Dichloro-o-anisic acid
(Dicamba) Herbicide 221
EtOH/
Water Water 2-8°C -20°C F 0.01-10.0 Callus
G001 Gibberellic acid (GA3
)
Plant
hormone 346.4
EtOH/ 1N
KOH Water 2-8°C 2-8°C CA/F 0.01-5.0 Elongation of internodes
G026 Gibberellic Acid (A4
+ 7)
Plant
hormone 332.4
EtOH/ 1N
KOH Water 2-8°C 2-8°C F 0.01-5.0 Elongation of internodes
GAL01
Gibberellic acid Solution
1mg/mL
Plant
hormone 2-8°C CA 0.01-5.0 Elongation of internodes
G032 Glyphosate Herbicide 169.07 1N KOH Water 2-8°C -20°C F 50-80 No growth for several weeks, then growth
P007 Phloroglucinol Other 126.1 EtOH Water 15-30°C 2-8°C CA/F 10-150 Shoot and leaf formation
P054 Paclobutrazol
Growth
retardant 293.8 DMSO Water 15-30°C 2-8°C CA/F 0.1-10.0 Shortened internodes
T017 Thidiazuron
Cytokininlike 220.2
DMSO/1N
KOH Water 2-8°C -20°C CA/F 0.1-10.0
Shoot and leaf proliferation , shortened
internodes
TDL01 Thidiazuron Solution 1mg/mL
Cytokininlike -20°C CA 0.1-10.0
Shoot and leaf proliferation , shortened
internodes

Catalog
No. Product Name Type Mol. Wt. Solvent Diluent
Powder
Storage
Liquid
Storage
Sterilization
Working
Conc.
(mg/L) Plant Effect
D001 2,4-Dichlorophenoxyacetic acid Auxin 221
EtOH/1N
KOH Water 15-30°C 2-8°C CA 0.01-6.0 Callus
DSL02
2,4-Dichlorophenoxyacetic acid
1mg/mL Solution Auxin 2-8°C CA 0.01-6.0 Callus
I001
Indole-3-acetic acid Free acid
(IAA) Auxin 175.2
EtOH/1N
KOH Water -20°C -20°C CA/F 0.01-3.0 Roots and callus
ISL01 IAA Solution 1mg/mL Auxin -20°C CA 0.01-3.0 Roots and callus
I002 Indole-3-butyric acid (IBA) Auxin 203.2
EtOH/1N
KOH Water 2-8°C -20°C CA/F 0.1-10.0 Roots and callus
I008
Indole-3-butyric acid Potassium
salt (IBA-K) Auxin 241.3 Water Water 2-8°C -20°C CA/F 0.1-10.0 Roots and callus
ISL02 IBA Solution 1mg/mL Auxin -20°C CA 0.1-10.0 Roots and callus
I009 3-Indolepropionic Acid (IPA) Auxin 189.21 1N KOH Water -20°C -20°C CA 0.1-10.0 Roots and callus
N001
Naphthaleneacetic acid Free
acid (NAA) Auxin 186.2 1N KOH Water 15-30°C 2-8°C CA 0.1-10.0 Callus
N010
Naphthaleneacetic acid Free
acid (NAA) Auxin 224.3 Water Water 15-30°C 2-8°C CA/F 0.1-10.0 Callus
NSL01 NAA Solution 1mg/mL Auxin 2-8°C CA 0.1-10.0 Callus
A025 Adenine Sulfate, Dihydrate Cytokinin 404.4
Warm
Water
Warm
Water 15-30°C 2-8°C CA 50-250 thickened shoots with leaves
B001 6-Benzylaminopurine (BAP) Cytokinin 225.3 1N KOH Water 15-30°C 2-8°C CA/F 0.1-5.0 Shoot and leaf proliferation
BSL01 BAP Solution 1mg/mL Cytokinin 2-8°C CA 0.1-5.0 Shoot and leaf proliferation
D006
6-(γ,γ-Dimethylallylamino)purine
(2iP) Cytokinin 203.2 1N KOH Water -20°C -20°C CA/F 1.0-30.0 Shoot and leaf proliferation
DSL01 2iP Solution 1mg/mL Cytokinin -20°C CA 1.0-30.0 Shoot and leaf proliferation
K001 Kinetin Cytokinin 215.2 1N KOH Water -20°C -20°C CA/F 0.1-5.0 Shoot and leaf proliferation
KSL01 Kinetin Solution 1mg/mL Cytokinin -20°C CA 0.1-5.0 Shoot and leaf proliferation
T039 Meta-Topolin Cytokinin 241.25 1N KOH Water -20°C -20°C CA/F 0.1-10.0 Shoot and leaf proliferation
Z007 trans-Zeatin Cytokinin 219.2 1N KOH Water -20°C -20°C CA/F 0.01-5.0 Shoot and leaf proliferation
Z008 trans-Zeatin Riboside Cytokinin 351.4 1N KOH Water -20°C -20°C F 0.01-5.0 Shoot and leaf proliferation
ZSL01 trans-Zeatin Solution 1mg/mL Cytokinin -20°C CA 0.01-5.0 Shoot and leaf proliferation